Ecosyste.ms: Issues

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GitHub / kalekundert/stepwise_mol_bio issues and pull requests

#44 - digest: use recommended reaction volume

Issue - State: open - Opened by kalekundert about 2 years ago

#42 - digest: option for −enzyme controls

Issue - State: open - Opened by kalekundert about 2 years ago - 1 comment

#41 - ligate: confusing error message if MW can't be found

Issue - State: open - Opened by kalekundert about 2 years ago

#40 - ligate: warn when using phosphorylated backbone

Issue - State: open - Opened by kalekundert about 2 years ago

#39 - digest: add phosphatase option

Issue - State: open - Opened by kalekundert about 2 years ago - 2 comments

#38 - ligate: option to *not* merge reactions

Issue - State: open - Opened by kalekundert about 2 years ago

#37 - direct/serial: calculate initial dilutions

Issue - State: open - Opened by kalekundert about 2 years ago

#36 - digest: directly specify volume of DNA

Issue - State: open - Opened by kalekundert about 2 years ago

#35 - Add full plasmid sequencing protocol

Issue - State: open - Opened by kalekundert about 2 years ago - 1 comment

#34 - Add electrotransformation protocol

Issue - State: open - Opened by kalekundert over 2 years ago
Labels: high priority

#33 - Add "unbiased" PCR protocol

Issue - State: open - Opened by kalekundert over 2 years ago
Labels: high priority

#32 - Think about how samples load the FreezerBox database

Issue - State: open - Opened by kalekundert over 2 years ago

#31 - thermocycler: allow steps to be named

Issue - State: open - Opened by kalekundert over 2 years ago

#30 - sequence: add way to specify colony PCR primers

Issue - State: open - Opened by kalekundert over 2 years ago

#28 - spin cleanup: warn if column capacity is exceeded

Issue - State: open - Opened by kalekundert over 2 years ago

#27 - pcr: allow annealing temperature to be a range

Issue - State: open - Opened by kalekundert over 2 years ago

#26 - Add an E-gel protocol

Issue - State: open - Opened by kalekundert over 2 years ago

#25 - Migrate to BYOC

Issue - State: closed - Opened by kalekundert over 2 years ago - 1 comment

#24 - Refactor unit tests

Issue - State: closed - Opened by kalekundert over 2 years ago - 1 comment

#23 - ethanol: read presets from a config file

Issue - State: open - Opened by kalekundert over 2 years ago

#22 - Add a trizol protocol

Issue - State: closed - Opened by kalekundert over 2 years ago - 1 comment

#21 - pcr: allow multiple products to be specified

Issue - State: open - Opened by kalekundert over 2 years ago
Labels: high priority

#20 - pcr: add an option to explain how Ta was determined

Issue - State: open - Opened by kalekundert over 2 years ago

#19 - Add a plate-pouring protocol

Issue - State: open - Opened by kalekundert over 2 years ago

#18 - digest: unnecessary denature step

Issue - State: closed - Opened by kalekundert almost 3 years ago - 2 comments

#17 - Universal config options

Issue - State: open - Opened by kalekundert about 3 years ago

#16 - Add an overlap extension PCR protocol

Issue - State: open - Opened by kalekundert about 3 years ago - 1 comment

#15 - pcr: add touchdown options

Issue - State: open - Opened by kalekundert about 3 years ago

#14 - pcr: require extension time

Issue - State: open - Opened by kalekundert about 3 years ago

#13 - Add a gel purification protocol

Issue - State: open - Opened by kalekundert about 3 years ago - 2 comments
Labels: high priority

#12 - Add a protocol for making buffers

Issue - State: open - Opened by kalekundert over 3 years ago - 1 comment

#11 - Set command-line defaults from FreezerBox database

Issue - State: closed - Opened by kalekundert over 3 years ago - 1 comment

#10 - Add a "miniprep" protocol

Issue - State: open - Opened by kalekundert over 3 years ago

#9 - gel: add pre-running options

Issue - State: closed - Opened by kalekundert over 3 years ago

#8 - Add a "transformation" protocol

Issue - State: closed - Opened by kalekundert over 3 years ago

#7 - ethanol: add some line breaks

Issue - State: open - Opened by kalekundert over 3 years ago

#6 - Add a generic thermocycler protocol.

Issue - State: closed - Opened by kalekundert almost 4 years ago - 1 comment

#5 - Be more clever about caching reactions

Issue - State: closed - Opened by kalekundert almost 4 years ago - 1 comment

#4 - digest: Add option to skip heat denaturation step

Issue - State: open - Opened by kalekundert about 4 years ago

#3 - ivt: Incorporate NEB recommendations for purifying the template

Issue - State: closed - Opened by kalekundert over 4 years ago - 1 comment

#2 - ivt: Allow the aliquot concentration and volume to be specified

Issue - State: closed - Opened by kalekundert over 4 years ago - 1 comment

#1 - ivt: don't allow negative solvent volumes

Issue - State: closed - Opened by kalekundert over 4 years ago - 1 comment